MicroRNA Stability in FFPE Tissue Samples: Dependence on GC Content
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چکیده
منابع مشابه
MicroRNA Stability in FFPE Tissue Samples: Dependence on GC Content
MicroRNAs (miRNAs) are small non-coding RNAs responsible for fine-tuning of gene expression at post-transcriptional level. The alterations in miRNA expression levels profoundly affect human health and often lead to the development of severe diseases. Currently, high throughput analyses, such as microarray and deep sequencing, are performed in order to identify miRNA biomarkers, using archival p...
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MicroRNAs (miRNAs) are very short (18-24 nucleotides) nucleic acids that are expressed in a number of biological tissues and have been shown to be more resistant to extreme temperatures and pH compared to longer RNA molecules, like mRNAs. As miRNAs contribute to diverse biological process and respond to various kinds of cellular stress, their utility as diagnostic biomarkers and/or therapeutic ...
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Introduction RNA silencing is a mechanism of gene regulation whereby small double-stranded RNAs (dsRNAs) of 21–18 nucleotides in length cause mRNA degradation, mRNA translation, or chromatin modification [1]. In the past years, more than 500 novel genes have been identified in animals and plants encoding small transcripts that can form small dsRNA hairpin structures [2–4]. From these hairpin pr...
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BACKGROUND RNA integrity is the essential factor that determines the accuracy of mRNA transcript measurements obtained with quantitative real-time reverse-transcription PCR (RT-qPCR), but evidence is clearly lacking on whether this conclusion also applies to microRNAs (miRNAs). We evaluated this issue by comparative analysis of the dependence of miRNA and mRNA measurements on RNA integrity in r...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2016
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0163125